C11 BODIPY 581/591
BODIPY 581/591 C11 是一種 BODIPY 氟硼熒衍生物，具有良好的光穩定性、低熒光偽影特質。BODIPY 581/591 C11 常用于活細胞內脂質過氧化和抗氧化性能的研究，或與羥基自由基反應、檢測鐵死亡 (ferroptosis)。BODIPY 581/591 C11 本身為紅色熒光 (還原型；Ex=581 nm, Em=591 nm)，與脂質結合后會產生綠色熒光 (氧化型；Ex=500 nm, Em=510 nm)。
此產品僅用于科學研究，我們不為任何個人用途提供產品和服務
-
包裝價格促銷價數量
-
200ug￥1625.00350.00- +
-
1mg￥2625.00998.00- +
- 貨號: ajci64572
- CAS: 217075-36-0
- 別名: C11-Bodipy 581/591;Bodipy-C11;Bodipy C11;脂質過氧化探針;脂質過氧化熒光探針；鐵死亡熒光探針
- 分子式: C30H34BF2N2O2?H
- 分子量: 504.4
- 純度: ＞99%
- 溶解度: 30mg/ml in DMSO
- 儲存: Store at -20°C
- 庫存: 現貨
Background
C11 BODIPY 581/591 is a lipid-soluble ratiometric fluorescent indicator of lipid oxidation.[1] Upon oxidation, its excitation maximum shifts from 581 to 500 nm and the emission maximum shifts from 591 to 510 nm. C11 BODIPY 581/591 has been used in biochemical assays and live cell applications.[1],[2]
Reference:
[1]. Pap, E.H.W., Drummen, G.P.C., Winter, V.J., et al. Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY581/591 FEBS Lett. 453(3), 278-282 (1999).
[2]. Naguib, Y.M.A. A fluorometric method for measurement of peroxyl radical scavenging activities of lipophilic antioxidants Anal. Biochem. 265(2), 290-298 (1998).
恭喜以下科學家使用我司C11 BODIPY 581/591發表SCI論文：
1.Advanced Healthcare Materials.doi:10.1002/adhm.202202150.（IF:11.0，Q1）
2.Journal of Controlled Release.doi:10.1016/j.jconrel.2023.01.035 （IF:11.467，Q1）
3.Neoplasma Vol.69, No.3, p.648–656, 2022.doi:10.4149/neo_2022_211103N1568
4.Research Square(2022).doi:10.21203/rs.3.rs-2381256/v1
5.CNS Neuroscience & Therapeutics.22 February 2023.doi:10.1111/cns.14159
6.Journal of Colloid And Interface Science.645 (2023) 882–894.doi:10.1016/j.jcis.2023.05.003
7.Nano letters.April 10, 2023.doi:10.1021/acs.nanolett.3c00365
9.Brain Research.Volume 1824, 1 February 2024, 148689.doi:10.1016/j.brainres.2023.148689
10.Journal of Colloid And Interface Science.20 November 2023.doi:10.1016/j.jcis.2023.11.119
11.J. Agric. Food Chem. 2023, 71, 17295?17307.doi:10.1021/acs.jafc.3c03409
Protocol
Measurement of neuron lipid peroxides:Lipid peroxides in neuron were measured by C11-BODIPY 581/591 (Amgicam, Wuhan, China; catalog number: ajci64572) staining. Briefly, cells were stained with BODIPY (5 μM) for 30 min at 37°C for 30 min in the dark. Then, cells were washed with PBS three times and observed immediately under a fluorescent microscopy. The fluorescence images were collected using a single rapid scan. In addition, we analyzed the levels of lipid peroxides by a fluorescence microplate reader. Cells were stained with BODIPY, washed with PBS and centrifugated for 5 min at 1000 ×g, the supernatant was removed, and the remaining cells were resolved with 1% Triton X-100. Fluorescence was analyzed at an excitation wavelength of 500nm and an emission wavelength of 510nm.
Reference:CNS Neuroscience & Therapeutics.22 February 2023.doi:10.1111/cns.14159
in vivo LPO accumulation:The mice were sacrificed and tumors were collected for cryosection. Tumor sections were stained with C11-BODIPY 581/591 (2.5 μM) at 37 ℃ for 30 min, and fixed with 10% paraformaldehyde at room temperature for 10 min. Then the tissue sections were stained with DAPI for 10 min. Finally, oxidized C11-BODIPY 581/591 was observed under a confocal microscope to evaluate in vivo LPO accumulation.
Reference:Neoplasma Vol.69, No.3, p.648–656, 2022.doi:10.4149/neo_2022_211103N1568
