DPPH(free radical)
DPPH (2,2-Diphenyl-1-picrylhydrazyl) 是一種穩定的自由基，可用于測量抗氧化劑的自由基清除活性。DPPH 中氮原子的奇數電子通過從抗氧化劑吸收氫原子而還原為相應的肼。DPPH 方法可用于水性和非極性有機溶劑中，并可用于檢查親水性和親脂性抗氧化劑。
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包裝價格促銷價數量
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500mg￥400.00200.00- +
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1g￥700.00350.00- +
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5g￥1500.001200.00- +
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10g￥2500.002000.00- +
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100g￥18000.000.00- +
- 貨號: ajci23192
- CAS: 1898-66-4
- 別名: 2,2-聯苯基-1-苦基肼基,2,2-Diphenyl-1-Picrylhydrazyl；DPPH radical
- 分子式: C18H12N5O6
- 分子量: 394.3
- 純度: ＞99%
- 溶解度: DMSO : 25 mg/mL (63.40 mM; Need ultrasonic)
- 儲存: Store at -20°C
- 庫存: 現貨
Background
DPPH, known formally as 2,2-diphenyl-1-picrylhydrazyl, is a cell-permeable, stable free radical that is commonly used to evaluate the ability of compounds to act as free radical scavengers or hydrogen donors and to measure the antioxidant activity of tissue extracts.[1] The reaction of DPPH with an antioxidant or reducing compound produces the corresponding hydrazine DPPH2, which can be followed by color change from purple (absorbance at 515-528 nm) to yellow. The details and limitations of analysis have been recently reviewed.[[2],[3],[4]
Reference:
[1]. Kedare, S.B., and Singh, R.P. Genesis and development of DPPH method of antioxidant assay J.Food Sci.Technol. 48(4), 412-422 (2011).
[2]. Molyneux, P. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity Songklanakarin J.Sci.Technol. 26(2), 211-219 (2004).
[3]. Sharma, O.P., and Bhat, T.K. DPPH antioxidant assay revisited Food Chemistry 113, 1202-1205 (2009).
[4]. Pyrzynska, K., and Pekal, A. Application of free radical diphenylpicrylhydrazyl (DPPH) to estimate the antioxidant capacity of food samples Anal.Methods 5, 4288-4295 (2013).
