2-NBDG
2-NBDG是一種熒光標(biāo)記的2-脫氧葡萄糖類似物，可用作細(xì)胞葡萄糖代謝評估的示蹤劑（激發(fā)/發(fā)射波長：475/550納米）。
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包裝價格促銷價數(shù)量
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5mg￥938.00450.00- +
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10mg￥1580.00850.00- +
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100mg￥11250.007500.00- +
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500mg￥25000.000.00- +
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1g￥42000.000.00- +
- 貨號: ajci4820
- CAS: 186689-07-6
- 別名: 2-(N-7-硝基-2,1,3-苯并惡二唑-4-氨基)-2-脫氧-D-葡萄糖；NBD-GLUCOSE;2-NBD-Glucose；葡萄糖轉(zhuǎn)運/血糖通道熒光探針
- 分子式: C12H14N4O8
- 分子量: 342.26
- 純度: ＞99%
- 溶解度: ≥17.1mg/mL in H2O with ultrasonic
- 儲存: Store at -20°C
- 庫存: 現(xiàn)貨
Background
葡萄糖是一種六碳單糖，通常以游離、結(jié)合的形式，存在于動植物體內(nèi).正常人血漿中葡萄糖含量在3.89~6.11mmol/L之間，尿液中一般不含游離葡萄糖，糖尿病患者尿中的含量變化比較大。葡萄糖是維持細(xì)胞活動和組織內(nèi)穩(wěn)態(tài)所必需的能量來源。糖代謝是許多疾病的重要靶點，并隨著病理條件的改變而改變，因此，糖代謝的評價可以作為疾病進(jìn)展的重要指標(biāo)。
2-NBDG是一種熒光標(biāo)記的2-脫氧葡萄糖類似物，所謂的類似是指與D型葡萄糖（D-glucose）具有相同的轉(zhuǎn)運體（GLUTs）和類似的胞內(nèi)代謝方式，與D型葡萄糖（D-glucose）競爭性抑制，可用作細(xì)胞葡萄糖代謝的示蹤劑。當(dāng)它被細(xì)胞快速攝取入胞內(nèi)，攝取程度與其孵育濃度在一定范圍內(nèi)成正相關(guān)，此過程一定時間的熒光強度反應(yīng)了葡萄糖吸收的情況，以此作為探針來測定葡萄糖。
2-NBDG可用于體外多種細(xì)胞，如HepG2人肝癌細(xì)胞、L6大鼠骨骼肌細(xì)胞、MCF-7乳腺癌上皮細(xì)胞和星形膠質(zhì)細(xì)胞，也可用于疾病模型、癲癇大鼠、高血糖、糖尿病或小鼠異種移植瘤模型。
2-NBDG的最大激發(fā)/發(fā)射波長為475/550nm，通過葡萄糖轉(zhuǎn)運蛋白進(jìn)入細(xì)胞，隨后被己糖激酶磷酸化并被困在細(xì)胞內(nèi)。流式細(xì)胞儀檢（FL1通道）測細(xì)胞產(chǎn)生的熒光，檢測細(xì)胞對2-NBDG的攝取，熒光微孔板法測定細(xì)胞內(nèi)轉(zhuǎn)運的2-NBDG濃度。它也可以用熒光成像顯微鏡（熒光素濾片F(xiàn)ITC）或CCD攝像機進(jìn)行檢測。
恭喜以下科學(xué)家使用我司2-NBDG發(fā)表文章：
1.Hindawi.Oxidative Medicine and Cellular Longevity.Volume 2022, Article ID 9148257, 24 pages
2.J. Agric. Food Chem. 2021, 69, 13838-13848
3.Free Radical Biology and Medicine.j.freeradbiomed.2022.09.029
4.Basic & Clinical Medicine.Vol.42 No.11.November 2022
5.Cancer Cell International volume 22, Article number: 143 (2022)
7.Biophysics Reports, 2023, 9(3): 134-145.2-NBDG,purchased from Amgicam.DOI:10.52601/bpr.2023.230003
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參考文獻(xiàn):
[1]. Zou C, Wang Y, Shen Z. 2-NBDG as a fluorescent indicator for direct glucose uptake measurement[J]. Journal of biochemical and biophysical methods, 2005, 64(3): 207-215.
[2]. O’Neil R G, Wu L, Mullani N. Uptake of a fluorescent deoxyglucose analog (2-NBDG) in tumor cells[J]. Molecular Imaging and Biology, 2005, 7(6): 388-392.
[3]. Tsytsarev V, Maslov K I, Yao J, et al. In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog[J]. Journal of neuroscience methods, 2012, 203(1): 136-140.
[4]. Yan Chen, Junjian Zhang, Xiang-yang Zhang, 2-NBDG as a Marker for Detecting Glucose Uptake in Reactive Astrocytes Exposed to Oxygen-Glucose Deprivation In Vitro. J Mol Neurosci (2015) 55:126–130.
[5]. Vassiliy Tsytsareva,b,1,2, Konstantin I. Maslova,1,3, Junjie Yaoa,1,3, et al, In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog, J Neurosci Methods. 2012 Jan 15;203(1):136-40.
Protocol
First, 100 μM of 2-NBDG (Amgicam, Wuhan, China) was added to the cells in each group, and then, the cells were incubated at 37 ℃ for 1h, washed with PBS three times, and finally observed under a fluorescence microscope. The mean fluorescence intensity values were analyzed using the ImageJ software.
References: 1.Free Radical Biology and Medicine.j.freeradbiomed.2022.09.029.DOI:10.1016/j.freeradbiomed.2022.09.029
